Ontology highlight
ABSTRACT:
SUBMITTER: Imai H
PROVIDER: S-EPMC3926671 | biostudies-literature | 2014 Mar
REPOSITORIES: biostudies-literature
Imai Hiroo H Kato Shunsuke S Sakamoto Yasuhiro Y Kakudo Yuichi Y Shimodaira Hideki H Ishioka Chikashi C
Oncology reports 20131230 3
The TP53 mutation (R175H) is one of the most common mutations in human cancer. It is a highly attractive strategy for cancer therapy to find the genes that lead the R175H-expressing cancer cells. The aim of this study was to identify the synthetic sick/lethal gene interacting with R175H. Using lentiviral bar-coded comprehensive shRNA library and a tetracycline-inducible R175H expressed in the SF126 human glioblastoma cell line (SF126-tet-R175H), we conducted high-throughput screening to identify ...[more]