Ontology highlight
ABSTRACT:
SUBMITTER: Kasinathan S
PROVIDER: S-EPMC3929178 | biostudies-literature | 2014 Feb
REPOSITORIES: biostudies-literature
Kasinathan Sivakanthan S Orsi Guillermo A GA Zentner Gabriel E GE Ahmad Kami K Henikoff Steven S
Nature methods 20131215 2
Sequence-specific DNA-binding proteins including transcription factors (TFs) are key determinants of gene regulation and chromatin architecture. TF profiling is commonly carried out by formaldehyde cross-linking and sonication followed by chromatin immunoprecipitation (X-ChIP). We describe a method to profile TF binding at high resolution without cross-linking. We begin with micrococcal nuclease-digested non-cross-linked chromatin and then perform affinity purification of TFs and paired-end sequ ...[more]