Ontology highlight
ABSTRACT:
SUBMITTER: Hens K
PROVIDER: S-EPMC3929264 | biostudies-literature | 2011 Oct
REPOSITORIES: biostudies-literature
Hens Korneel K Feuz Jean-Daniel JD Isakova Alina A Iagovitina Antonina A Massouras Andreas A Bryois Julien J Callaerts Patrick P Celniker Susan E SE Deplancke Bart B
Nature methods 20111030 12
Drosophila melanogaster has one of the best characterized metazoan genomes in terms of functionally annotated regulatory elements. To explore how these elements contribute to gene regulation, we need convenient tools to identify the proteins that bind to them. Here we describe the development and validation of a high-throughput yeast one-hybrid platform, which enables screening of DNA elements versus an array of full-length, sequence-verified clones containing over 85% of predicted Drosophila tr ...[more]