Unknown

Dataset Information

0

Teneurin-4 promotes cellular protrusion formation and neurite outgrowth through focal adhesion kinase signaling.


ABSTRACT: Teneurin-4 (Ten-4), a transmembrane protein, is highly expressed in the central nervous system; however, its cellular and molecular function in neuronal differentiation remains unknown. In this study, we aimed to elucidate the function of Ten-4 in neurite outgrowth. Ten-4 expression was induced during neurite outgrowth of the neuroblastoma cell line Neuro-2a. Ten-4 protein was localized at the neurite growth cones. Knockdown of Ten-4 expression in Neuro-2a cells decreased the formation of the filopodia-like protrusions and the length of individual neurites. Conversely, overexpression of Ten-4 promoted filopodia-like protrusion formation. In addition, knockdown and overexpression of Ten-4 reduced and elevated the activation of focal adhesion kinase (FAK) and Rho-family small GTPases, Cdc42 and Rac1, key molecules for the membranous protrusion formation downstream of FAK, respectively. Inhibition of the activation of FAK and neural Wiskott-Aldrich syndrome protein (N-WASP), which is a downstream regulator of FAK and Cdc42, blocked protrusion formation by Ten-4 overexpression. Further, Ten-4 colocalized with phosphorylated FAK in the filopodia-like protrusion regions. Together, our findings show that Ten-4 is a novel positive regulator of cellular protrusion formation and neurite outgrowth through the FAK signaling pathway.

SUBMITTER: Suzuki N 

PROVIDER: S-EPMC3929675 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature

altmetric image

Publications

Teneurin-4 promotes cellular protrusion formation and neurite outgrowth through focal adhesion kinase signaling.

Suzuki Nobuharu N   Numakawa Tadahiro T   Chou Joshua J   de Vega Susana S   Mizuniwa Chihiro C   Sekimoto Kaori K   Adachi Naoki N   Kunugi Hiroshi H   Arikawa-Hirasawa Eri E   Yamada Yoshihiko Y   Akazawa Chihiro C  

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 20131216 3


Teneurin-4 (Ten-4), a transmembrane protein, is highly expressed in the central nervous system; however, its cellular and molecular function in neuronal differentiation remains unknown. In this study, we aimed to elucidate the function of Ten-4 in neurite outgrowth. Ten-4 expression was induced during neurite outgrowth of the neuroblastoma cell line Neuro-2a. Ten-4 protein was localized at the neurite growth cones. Knockdown of Ten-4 expression in Neuro-2a cells decreased the formation of the fi  ...[more]

Similar Datasets

| S-EPMC3635460 | biostudies-literature
| S-EPMC11352776 | biostudies-literature
| S-EPMC6549137 | biostudies-literature
| S-EPMC3767638 | biostudies-literature
| S-EPMC2602877 | biostudies-literature
| S-EPMC3623789 | biostudies-literature
| S-EPMC139731 | biostudies-literature
| S-EPMC2266630 | biostudies-literature
| S-EPMC124153 | biostudies-literature
2013-09-17 | GSE43873 | GEO