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Electrophysiology of human cardiac atrial and ventricular telocytes.


ABSTRACT: Telocytes (TCs) with exceptionally long cellular processes of telopodes have been described in human epicardium to act as structural supporting cells in the heart. We examined myocardial chamber-specific TCs identified in atrial and ventricular fibroblast culture using immunocytochemistry and studied their electrophysiological property by whole-cell patch clamp. Atrial and ventricular TCs with extended telopodes and alternating podoms and podomers that expressed CD34, c-Kit and PDGFR-? were identified. These cells expressed large conductance Ca²?-activated K? current (BK(Ca)) and inwardly rectifying K? current (IK(ir)), but not transient outward K? current (I(to)) and ATP-sensitive potassium current (K(ATP)). The active channels were functionally competent with demonstrated modulatory response to H2 S and transforming growth factor (TGF)-?1 whereby H?S significantly inhibited the stimulatory effect of TGF-?1 on current density of both BKCa and IK(ir). Furthermore, H?S attenuated TGF-?1-stimulated KCa1.1/Kv1.1 (encode BK(Ca)) and Kir2.1 (encode IK(ir)) expression in TCs. Our results show that functionally competent K? channels are present in human atrial and ventricular TCs and their modulation may have significant implications in myocardial physiopathology.

SUBMITTER: Sheng J 

PROVIDER: S-EPMC3930421 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature

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Electrophysiology of human cardiac atrial and ventricular telocytes.

Sheng Jingwei J   Shim Winston W   Lu Jun J   Lim Sze Yun SY   Ong Boon Hean BH   Lim Tien Siang TS   Liew Reginald R   Chua Yeow Leng YL   Wong Philip P  

Journal of cellular and molecular medicine 20140201 2


Telocytes (TCs) with exceptionally long cellular processes of telopodes have been described in human epicardium to act as structural supporting cells in the heart. We examined myocardial chamber-specific TCs identified in atrial and ventricular fibroblast culture using immunocytochemistry and studied their electrophysiological property by whole-cell patch clamp. Atrial and ventricular TCs with extended telopodes and alternating podoms and podomers that expressed CD34, c-Kit and PDGFR-β were iden  ...[more]

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