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Expression, purification and crystallization of a dye-decolourizing peroxidase from Dictyostelium discoideum.

ABSTRACT: Dye-decolourizing peroxidases are haem-containing peroxidases with broad substrate specificity. Using H2O2 as an electron acceptor, they efficiently decolourize various dyes that are of industrial and environmental relevance, such as anthraquninone- and azo-based dyes. In this study, the dye-decolourizing peroxidase DdDyP from Dictyostelium discoideum was overexpressed in Escherichia coli strain Rosetta(DE3)pLysS, purified and crystallized using the vapour-diffusion method. A native crystal diffracted to 1.65 Å resolution and belonged to space group P4(1)2(1)2, with unit-cell parameters a = b = 141.03, c = 95.56 Å, ? = ? = ? = 90°. The asymmetric unit contains two molecules.

SUBMITTER: Rai A

PROVIDER: S-EPMC3936452 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature

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Expression, purification and crystallization of a dye-decolourizing peroxidase from Dictyostelium discoideum.

Rai Amrita A Fedorov Roman R Manstein Dietmar J DJ

Acta crystallographica. Section F, Structural biology communications 20140122 Pt 2

Dye-decolourizing peroxidases are haem-containing peroxidases with broad substrate specificity. Using H2O2 as an electron acceptor, they efficiently decolourize various dyes that are of industrial and environmental relevance, such as anthraquninone- and azo-based dyes. In this study, the dye-decolourizing peroxidase DdDyP from Dictyostelium discoideum was overexpressed in Escherichia coli strain Rosetta(DE3)pLysS, purified and crystallized using the vapour-diffusion method. A native crystal diff ...[more]

PMID: 24637768

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