Ontology highlight
ABSTRACT:
SUBMITTER: Hu G
PROVIDER: S-EPMC3938014 | biostudies-literature | 2011 Sep
REPOSITORIES: biostudies-literature
Hu Gui G Goll Mary G MG Fisher Shannon S
Developmental dynamics : an official publication of the American Association of Anatomists 20110729 9
Site-specific recombinases (SSRs) are powerful tools for genome manipulation, used in diverse organisms including Drosophila melanogaster, mouse, Arabidopsis, zebrafish, and human cultured cells. The integrase from the bacteriophage ΦC31 belongs to the large serine family of integrases, and in contrast to other widely used SSRs such as Cre and Flp, recombination is directional and therefore irreversible. We have developed a vector system for recombinase-mediated cassette exchange (RMCE) in the z ...[more]