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Global transcriptome profiles of Italian Mediterranean buffalo embryos with normal and retarded growth.

ABSTRACT: The transcriptome profiles were compared for buffalo embryos with normal growth and embryos with retarded growth on Day 25 after mating. Embryos with retarded growth on Day 25 after mating have a reduced likelihood of undergoing attachment to the uterine endometrium and establishing a pregnancy. Italian Mediterranean buffaloes were mated by AI and on Day 25 underwent trans-rectal ultrasonography to ascertain embryo development. Embryos with an embryonic width (EW)>2.7 mm were classed as normal embryos and embryos with an EW<2.7 mm were classed as retarded embryos. Three buffaloes with embryos of the largest EW (3.7, 3.7 and 3.9 mm) and three buffaloes with embryos of the smallest EW (1.5, 1.6 and 1.9 mm) were slaughtered on Day 27 to recover embryos for transcriptome analysis using a bovine custom designed oligo array. A total of 1,047 transcripts were differentially expressed between embryos with normal growth and embryos with retarded growth. Retarded embryos showed 773/1,047 (74%) transcripts that were down-regulated and 274/1,047 (26%) transcripts that were up-regulated relative to normal embryos; in silico analyses focused on 680/1,047 (65%) of the differentially expressed transcripts. The most altered transcripts observed in retarded embryos were associated with membrane structure and function and with metabolic and homeostasis maintenance functions. Other notable functions altered in retarded embryos were developmental processes and in particular nervous system differentiation and function. Specific biochemical pathways such as the complement cascade and coagulation were also altered in retarded embryos. It was concluded from the findings that buffalo embryos with retarded growth on Day 25 after mating show altered gene expression compared with normal embryos, and some de-regulated functions are associated with attachment to the uterine endometrium.

SUBMITTER: Strazzullo M

PROVIDER: S-EPMC3938533 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature

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Global transcriptome profiles of Italian Mediterranean buffalo embryos with normal and retarded growth.

Strazzullo Maria M Gasparrini Bianca B Neglia Gianluca G Balestrieri Maria Luisa ML Francioso Romina R Rossetti Cristina C Nassa Giovanni G De Filippo Maria Rosaria MR Weisz Alessandro A Di Francesco Serena S Vecchio Domenico D D'Esposito Maurizio M D'Occhio Michael John MJ Zicarelli Luigi L Campanile Giuseppe G

PloS one 20140228 2

The transcriptome profiles were compared for buffalo embryos with normal growth and embryos with retarded growth on Day 25 after mating. Embryos with retarded growth on Day 25 after mating have a reduced likelihood of undergoing attachment to the uterine endometrium and establishing a pregnancy. Italian Mediterranean buffaloes were mated by AI and on Day 25 underwent trans-rectal ultrasonography to ascertain embryo development. Embryos with an embryonic width (EW)>2.7 mm were classed as normal e ...[more]

PMID: 24587197

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Dataset Information

Global transcriptome profiles of Italian Mediterranean buffalo embryos with normal and retarded growth.

Publications

Global transcriptome profiles of Italian Mediterranean buffalo embryos with normal and retarded growth.

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