Gene expression profiling of human kidneys undergoing laparoscopic donor nephrectomy.

ABSTRACT:

Background and objectives

The objective was to compare gene expression profiles of 6 kidneys from open donor nephrectomy with 6 kidneys removed after laparoscopic donor nephrectomy and several hours of carbon dioxide pneumoperitoneum with DNA microarrays and identify small-molecule drugs.

Methods

The gene expression profile GSE3297 was downloaded from the Gene Expression Omnibus database, and the differentially expressed genes were identified by a bioinformatics approach. First, Osprey software was used to construct a differentially expressed gene associated network. Then, DAVID (Database for Annotation, Visualization, and Integrated Discovery) and FuncAssociate were used to perform functional analyses. Finally, the Connectivity Map was used to screen for small-molecule drug

SUBMITTER: Xu YH 

PROVIDER: S-EPMC3939323 | biostudies-literature | 2014 Jan-Mar

REPOSITORIES: biostudies-literature