Unknown

Dataset Information

0

Structure of the SAS-6 cartwheel hub from Leishmania major.


ABSTRACT: Centrioles are cylindrical cell organelles with a ninefold symmetric peripheral microtubule array that is essential to template cilia and flagella. They are built around a central cartwheel assembly that is organized through homo-oligomerization of the centriolar protein SAS-6, but whether SAS-6 self-assembly can dictate cartwheel and thereby centriole symmetry is unclear. Here we show that Leishmania major SAS-6 crystallizes as a 9-fold symmetric cartwheel and provide the X-ray structure of this assembly at a resolution of 3.5 Å. We furthermore demonstrate that oligomerization of Leishmania SAS-6 can be inhibited by a small molecule in vitro and provide indications for its binding site. Our results firmly establish that SAS-6 can impose cartwheel symmetry on its own and indicate how this process might occur mechanistically in vivo. Importantly, our data also provide a proof-of-principle that inhibition of SAS-6 oligomerization by small molecules is feasible. DOI: http://dx.doi.org/10.7554/eLife.01812.001.

SUBMITTER: van Breugel M 

PROVIDER: S-EPMC3939493 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature

altmetric image

Publications

Structure of the SAS-6 cartwheel hub from Leishmania major.

van Breugel Mark M   Wilcken Rainer R   McLaughlin Stephen H SH   Rutherford Trevor J TJ   Johnson Christopher M CM  

eLife 20140101


Centrioles are cylindrical cell organelles with a ninefold symmetric peripheral microtubule array that is essential to template cilia and flagella. They are built around a central cartwheel assembly that is organized through homo-oligomerization of the centriolar protein SAS-6, but whether SAS-6 self-assembly can dictate cartwheel and thereby centriole symmetry is unclear. Here we show that Leishmania major SAS-6 crystallizes as a 9-fold symmetric cartwheel and provide the X-ray structure of thi  ...[more]

Similar Datasets

| S-EPMC3655416 | biostudies-literature
| S-EPMC4116473 | biostudies-literature
| S-EPMC9592557 | biostudies-literature
| S-EPMC3388911 | biostudies-literature
| PRJNA624043 | ENA
| PRJNA242762 | ENA
| PRJNA9528 | ENA
| PRJNA788482 | ENA
| PRJNA259659 | ENA
| PRJNA110061 | ENA