Ontology highlight
ABSTRACT:
SUBMITTER: Kohler AC
PROVIDER: S-EPMC3940199 | biostudies-literature | 2014 Feb
REPOSITORIES: biostudies-literature
Kohler Amanda C AC Spanò Stefania S Galán Jorge E JE Stebbins C Erec CE
Acta crystallographica. Section D, Biological crystallography 20140129 Pt 2
GtgE is an effector protein from Salmonella Typhimurium that modulates trafficking of the Salmonella-containing vacuole. It exerts its function by cleaving the Rab-family GTPases Rab29, Rab32 and Rab38, thereby preventing the delivery of antimicrobial factors to the bacteria-containing vacuole. Here, the crystal structure of GtgE at 1.65 Å resolution is presented, and structure-based mutagenesis and in vivo infection assays are used to identify its catalytic triad. A panel of cysteine protease i ...[more]