Rotamer strain as a determinant of protein structural specificity.
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ABSTRACT:
SUBMITTER: Lazar GA
PROVIDER: S-EPMC2144231 | biostudies-other | 1999 Dec
REPOSITORIES: biostudies-other
Publications
Rotamer strain as a determinant of protein structural specificity.
Lazar G A GA Johnson E C EC Desjarlais J R JR Handel T M TM
Protein science : a publication of the Protein Society 19991201 12
We present direct evidence for a change in protein structural specificity due to hydrophobic core packing. High resolution structural analysis of a designed core variant of ubiquitin reveals that the protein is in slow exchange between two conformations. Examination of side-chain rotamers indicates that this dynamic response and the lower stability of the protein are coupled to greater strain and mobility in the core. The results suggest that manipulating the level of side-chain strain may be on ...[more]
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