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A versatile ultra-high performance LC-MS method for lipid profiling.

ABSTRACT: A new UPLC-based untargeted lipidomic approach using a qTOF hybrid mass spectrometer is introduced. The applied binary gradient enables separations of lipid species including constitutional isomeric compounds and low abundant lipid classes such as phosphatidic acid (PA). Addition of phosphoric acid to the solvents improves peak shapes for acidic phospholipids. MS(E) scans allow simultaneous acquisition of full scan data and collision induced fragmentation to improve identification of lipid classes and to obtain structural information. The method was used to investigate the lipidome of yeast.

SUBMITTER: Knittelfelder OL

PROVIDER: S-EPMC3946075 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature

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A versatile ultra-high performance LC-MS method for lipid profiling.

Knittelfelder Oskar L OL Weberhofer Bernd P BP Eichmann Thomas O TO Kohlwein Sepp D SD Rechberger Gerald N GN

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences 20140129

A new UPLC-based untargeted lipidomic approach using a qTOF hybrid mass spectrometer is introduced. The applied binary gradient enables separations of lipid species including constitutional isomeric compounds and low abundant lipid classes such as phosphatidic acid (PA). Addition of phosphoric acid to the solvents improves peak shapes for acidic phospholipids. MS(E) scans allow simultaneous acquisition of full scan data and collision induced fragmentation to improve identification of lipid class ...[more]

PMID: 24548922

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