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Large scale screening of human sera for HCV RNA and GBV-C RNA.

ABSTRACT: North Carolina locates acute HIV cases by pooled nucleic acid testing of HIV-antibody negative serum samples. Here, 224 pools of 80 HIV-negative samples (N = 17,920) were screened for viral RNA from HCV, GBV-C, and influenza A. No evidence of influenza A was found, but HCV and GBV-C were common (1.2% and 1.7% prevalence, respectively), demonstrating the utility of pooled testing in locating individuals that may remain undiagnosed otherwise. By sequencing positive pools, potential transmission clusters may be located as well.

SUBMITTER: Keys JR

PROVIDER: S-EPMC3947266 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature

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Large scale screening of human sera for HCV RNA and GBV-C RNA.

Keys Jessica R JR Leone Peter A PA Eron Joseph J JJ Alexander Kelcie K Brinson Myra M Swanstrom Ronald R

Journal of medical virology 20131031 3

North Carolina locates acute HIV cases by pooled nucleic acid testing of HIV-antibody negative serum samples. Here, 224 pools of 80 HIV-negative samples (N = 17,920) were screened for viral RNA from HCV, GBV-C, and influenza A. No evidence of influenza A was found, but HCV and GBV-C were common (1.2% and 1.7% prevalence, respectively), demonstrating the utility of pooled testing in locating individuals that may remain undiagnosed otherwise. By sequencing positive pools, potential transmission cl ...[more]

PMID: 24178362

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