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Novel tripod amphiphiles for membrane protein analysis.

ABSTRACT: Integral membrane proteins play central roles in controlling the flow of information and molecules across membranes. Our understanding of membrane protein structures and functions, however, is seriously limited, mainly due to difficulties in handling and analysing these proteins in aqueous solution. The use of a detergent or other amphipathic agents is required to overcome the intrinsic incompatibility between the large lipophilic surfaces displayed by the membrane proteins in their native forms and the polar solvent molecules. Here, we introduce new tripod amphiphiles displaying favourable behaviours toward several membrane protein systems, leading to an enhanced protein solubilisation and stabilisation compared to both conventional detergents and previously described tripod amphiphiles.

SUBMITTER: Chae PS

PROVIDER: S-EPMC3947462 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature

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Novel tripod amphiphiles for membrane protein analysis.

Chae Pil Seok PS Kruse Andrew C AC Gotfryd Kamil K Rana Rohini R RR Cho Kyung Ho KH Rasmussen Søren G F SG Bae Hyoung Eun HE Chandra Richa R Gether Ulrik U Guan Lan L Kobilka Brian K BK Loland Claus J CJ Byrne Bernadette B Gellman Samuel H SH

Chemistry (Weinheim an der Bergstrasse, Germany) 20131002 46

Integral membrane proteins play central roles in controlling the flow of information and molecules across membranes. Our understanding of membrane protein structures and functions, however, is seriously limited, mainly due to difficulties in handling and analysing these proteins in aqueous solution. The use of a detergent or other amphipathic agents is required to overcome the intrinsic incompatibility between the large lipophilic surfaces displayed by the membrane proteins in their native forms ...[more]

PMID: 24123610

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