Ontology highlight
ABSTRACT: Objectives
Glucokinase encoded by GCK is a key enzyme that facilitates phosphorylation of glucose to glucose-6-phosphate. Variants of GCK gene were shown to be associated with type 2 diabetes (T2D) and coronary heart disease (CHD). The goal of this study was to investigate the contribution of GCK gene-body methylation to the risk of CHD.Design and methods
36 patients (18 males and 18 females) and 36 age- and sex-matched controls were collected for the current methylation research. DNA methylation level of the CpG island (CGI) region on the GCK gene-body was measured through the sodium bisulfite DNA conversion and pyrosequencing technology.Results
Our results indicated that CHD cases have a much lower methylation level (49.77 ± 6.43%) compared with controls (54.47 ± 7.65%, P = 0.018). In addition, GCK gene-body methylation was found to be positively associated with aging in controls (r = 0.443, P = 0.010).Conclusions
Our study indicated that the hypomethylation of GCK gene-body was significantly associated with the risk of CHD. Aging correlates with an elevation of GCK methylation in healthy controls.
SUBMITTER: Xu L
PROVIDER: S-EPMC3947703 | biostudies-literature | 2014
REPOSITORIES: biostudies-literature
Xu Limin L Zheng Dawei D Wang Lingyan L Jiang Danjie D Liu Haibo H Xu Leiting L Liao Qi Q Zhang Lina L Liu Panpan P Shi Xinbao X Wang Zhaoyang Z Sun Lebo L Zhou Qingyun Q Li Ni N Huang Yi Y Le Yanping Y Ye Meng M Shao Guofeng G Duan Shiwei S
BioMed research international 20140217
<h4>Objectives</h4>Glucokinase encoded by GCK is a key enzyme that facilitates phosphorylation of glucose to glucose-6-phosphate. Variants of GCK gene were shown to be associated with type 2 diabetes (T2D) and coronary heart disease (CHD). The goal of this study was to investigate the contribution of GCK gene-body methylation to the risk of CHD.<h4>Design and methods</h4>36 patients (18 males and 18 females) and 36 age- and sex-matched controls were collected for the current methylation research ...[more]