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Phycobilisomes supply excitations to both photosystems in a megacomplex in cyanobacteria.


ABSTRACT: In photosynthetic organisms, photons are captured by light-harvesting antenna complexes, and energy is transferred to reaction centers where photochemical reactions take place. We describe here the isolation and characterization of a fully functional megacomplex composed of a phycobilisome antenna complex and photosystems I and II from the cyanobacterium Synechocystis PCC 6803. A combination of in vivo protein cross-linking, mass spectrometry, and time-resolved spectroscopy indicates that the megacomplex is organized to facilitate energy transfer but not intercomplex electron transfer, which requires diffusible intermediates and the cytochrome b6f complex. The organization provides a basis for understanding how phycobilisomes transfer excitation energy to reaction centers and how the energy balance of two photosystems is achieved, allowing the organism to adapt to varying ecophysiological conditions.

SUBMITTER: Liu H 

PROVIDER: S-EPMC3947847 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature

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Phycobilisomes supply excitations to both photosystems in a megacomplex in cyanobacteria.

Liu Haijun H   Zhang Hao H   Niedzwiedzki Dariusz M DM   Prado Mindy M   He Guannan G   Gross Michael L ML   Blankenship Robert E RE  

Science (New York, N.Y.) 20131101 6162


In photosynthetic organisms, photons are captured by light-harvesting antenna complexes, and energy is transferred to reaction centers where photochemical reactions take place. We describe here the isolation and characterization of a fully functional megacomplex composed of a phycobilisome antenna complex and photosystems I and II from the cyanobacterium Synechocystis PCC 6803. A combination of in vivo protein cross-linking, mass spectrometry, and time-resolved spectroscopy indicates that the me  ...[more]

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