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Inducible control of gene expression with destabilized Cre.

ABSTRACT: Acute manipulation of gene and protein function in the brain is essential for understanding the mechanisms of nervous system development, plasticity and information processing. Here we describe a technique based on a destabilized Cre recombinase (DD-Cre) whose activity is controlled by the antibiotic trimethoprim (TMP). We show that DD-Cre triggers rapid TMP-dependent recombination of loxP-flanked ('floxed') alleles in mouse neurons in vivo and validate the use of this system for neurobehavioral research.

SUBMITTER: Sando R

PROVIDER: S-EPMC3947879 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature

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Inducible control of gene expression with destabilized Cre.

Sando Richard R Baumgaertel Karsten K Pieraut Simon S Torabi-Rander Nina N Wandless Thomas J TJ Mayford Mark M Maximov Anton A

Nature methods 20130922 11

Acute manipulation of gene and protein function in the brain is essential for understanding the mechanisms of nervous system development, plasticity and information processing. Here we describe a technique based on a destabilized Cre recombinase (DD-Cre) whose activity is controlled by the antibiotic trimethoprim (TMP). We show that DD-Cre triggers rapid TMP-dependent recombination of loxP-flanked ('floxed') alleles in mouse neurons in vivo and validate the use of this system for neurobehavioral ...[more]

PMID: 24056874

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