Inducible control of gene expression with destabilized Cre.

ABSTRACT: Acute manipulation of gene and protein function in the brain is essential for understanding the mechanisms of nervous system development, plasticity and information processing. Here we describe a technique based on a destabilized Cre recombinase (DD-Cre) whose activity is controlled by the antibiotic trimethoprim (TMP). We show that DD-Cre triggers rapid TMP-dependent recombination of loxP-flanked ('floxed') alleles in mouse neurons in vivo and validate the use of this system for neurobehavioral research.

SUBMITTER: Sando R 

PROVIDER: S-EPMC3947879 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature