RNA replicon delivery via lipid-complexed PRINT protein particles.
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ABSTRACT:
SUBMITTER: Xu J
PROVIDER: S-EPMC3948333 | biostudies-literature | 2013 Sep
REPOSITORIES: biostudies-literature
Publications
RNA replicon delivery via lipid-complexed PRINT protein particles.
Xu Jing J Luft J Christopher JC Yi Xianwen X Tian Shaomin S Owens Gary G Wang Jin J Johnson Ashley A Berglund Peter P Smith Jonathan J Napier Mary E ME DeSimone Joseph M JM
Molecular pharmaceutics 20130821 9
Herein we report the development of a nonviral lipid-complexed PRINT (particle replication in nonwetting templates) protein particle system (LPP particle) for RNA replicon delivery with a view toward RNA replicon-based vaccination. Cylindrical bovine serum albumin (BSA) particles (diameter (d) 1 μm, height (h) 1 μm) loaded with RNA replicon and stabilized with a fully reversible disulfide cross-linker were fabricated using PRINT technology. Highly efficient delivery of the particles to Vero cell ...[more]
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