The effects of polymorphisms on human gene targeting.
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ABSTRACT:
SUBMITTER: Deyle DR
PROVIDER: S-EPMC3950700 | biostudies-literature | 2014 Mar
REPOSITORIES: biostudies-literature
Publications
The effects of polymorphisms on human gene targeting.
Deyle David R DR Li Li B LB Ren Gaoying G Russell David W DW
Nucleic acids research 20131225 5
DNA mismatches that occur between vector homology arms and chromosomal target sequences reduce gene targeting frequencies in several species; however, this has not been reported in human cells. Here we demonstrate that even a single mismatched base pair can significantly decrease human gene targeting frequencies. In addition, we show that homology arm polymorphisms can be used to direct allele-specific targeting or to improve unfavorable vector designs that introduce deletions. ...[more]
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