Unknown

Dataset Information

0

Obesity is associated with higher 4E-BP1 expression in endometrial cancer.


ABSTRACT:

Purpose

Obesity is associated with risk and prognosis of endometrial cancer (EC), and the mammalian target of rapamycin complex 1 (mTORC1) pathway may play an instrumental role. We sought to explore the associations between cellular proliferation, Akt, and 4E binding protein-1 (4E-BP1) (a downstream target of mTORC1), in obese and nonobese women with and without EC.

Methods

Archival tissue-specimens from endometrial biopsies were grouped into two broad categories based on the observed disease behavior and similarities in tissue staining patterns: benign/hyperplasia (without cytologic atypia) (n=18) versus atypia (complex hyperplasia with cytologic atypia)/carcinoma (n=25). The characteristics of the study population, including height and weight to determine body mass index (BMI: kg/m2), were abstracted from medical records. Immunohistochemistry was used to assess the phosphorylated (p)Akt, p4E-BP1, and antigen Ki67.

Results

Cytoplasmic and nuclear pAkt were significantly associated with cytoplasmic p4E-BP1 (ρ=+0.48, ρ=+0.50) (P<0.05) and nuclear p4E-BP1 (ρ=+0.40, ρ=+0.44) (P<0.05); cytoplasmic and nuclear p4E-BP1 were significantly associated with Ki67 (ρ=+0.46, ρ=+0.59) (P<0.05). Compared with the benign/hyperplasia group, the women with atypia/carcinoma had significantly higher cytoplasmic and nuclear p4E-BP1 and Ki67. This staining pattern was similar in obese women; however, in nonobese women, neither cytoplasmic nor nuclear p4E-BP1staining differed between benign/hyperplasia versus atypia/carcinoma.

Conclusion

The activation of 4E-BP1 was higher in the obese women with EC. Adiposity may be a key factor to consider in future studies investigating the role of 4E-BP1 as a biomarker and therapeutic target in EC.

SUBMITTER: Libby EF 

PROVIDER: S-EPMC3955094 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature

altmetric image

Publications

Obesity is associated with higher 4E-BP1 expression in endometrial cancer.

Libby Emily Falk EF   Azrad Maria M   Novak Lea L   Vazquez Ana I AI   Wilson Tamara R TR   Demark-Wahnefried Wendy W  

Current biomarker findings 20140101 4


<h4>Purpose</h4>Obesity is associated with risk and prognosis of endometrial cancer (EC), and the mammalian target of rapamycin complex 1 (mTORC1) pathway may play an instrumental role. We sought to explore the associations between cellular proliferation, Akt, and 4E binding protein-1 (4E-BP1) (a downstream target of mTORC1), in obese and nonobese women with and without EC.<h4>Methods</h4>Archival tissue-specimens from endometrial biopsies were grouped into two broad categories based on the obse  ...[more]

Similar Datasets

| S-EPMC4988876 | biostudies-literature
| S-EPMC1783830 | biostudies-literature
| S-EPMC6596930 | biostudies-literature
| S-EPMC3995540 | biostudies-literature
| S-EPMC4563739 | biostudies-literature
| S-EPMC10004604 | biostudies-literature
| S-EPMC4273997 | biostudies-literature
| S-EPMC4459815 | biostudies-literature
| S-EPMC9128821 | biostudies-literature
| S-EPMC5462087 | biostudies-literature