Immobilization of actively thromboresistant assemblies on sterile blood-contacting surfaces.
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ABSTRACT:
SUBMITTER: Qu Z
PROVIDER: S-EPMC3955951 | biostudies-literature | 2014 Jan
REPOSITORIES: biostudies-literature
Publications
Immobilization of actively thromboresistant assemblies on sterile blood-contacting surfaces.
Qu Zheng Z Krishnamurthy Venkat V Haller Carolyn A CA Dorr Brent M BM Marzec Ulla M UM Hurst Sawan S Hinds Monica T MT Hanson Stephen R SR Liu David R DR Chaikof Elliot L EL
Advanced healthcare materials 20130621 1
Rapid one-step modification of thrombomodulin with alkylamine derivatives such as azide, biotin, and PEG is achieved using an evolved sortase (eSrtA) mutant. The feasibility of a point-of-care scheme is demonstrated herein to site-specifically immobilize azido-thrombomodulin on sterilized commercial ePTFE vascular grafts, which exhibit superior thromboresistance compared with commercial heparin-coated grafts in a primate model of acute graft thrombosis. ...[more]
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