Characterization of a novel melt curve by use of the Roche LightCycler HSV 1/2 analyte-specific reagent real-time PCR assay: frequencies of this novel (low) melt curve and commonly encountered (intermediate) melt curves.
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ABSTRACT:
SUBMITTER: Boyanton BL
PROVIDER: S-EPMC3957778 | biostudies-literature | 2014 Mar
REPOSITORIES: biostudies-literature
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Characterization of a novel melt curve by use of the Roche LightCycler HSV 1/2 analyte-specific reagent real-time PCR assay: frequencies of this novel (low) melt curve and commonly encountered (intermediate) melt curves.
Boyanton Bobby L BL Almradi Amro A Espy Mark J MJ Prada Anne E AE Gibson John P JP Pritt Bobbi S BS
Journal of clinical microbiology 20131218 3
We characterize a novel probe binding-site polymorphism detectable solely by melt curve analysis using the Roche LightCycler HSV 1/2 analyte-specific reagent real-time PCR assay. The frequencies of this novel (47°C) and previously described intermediate (60 to 62°C) melt curves were 0.016% and 4.9%, respectively. ...[more]
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