Ontology highlight
ABSTRACT:
SUBMITTER: Zou J
PROVIDER: S-EPMC3957939 | biostudies-literature | 2014 Mar
REPOSITORIES: biostudies-literature
Journal of virology 20140103 6
<h4>Unlabelled</h4>Flavivirus replication is mediated by a complex machinery that consists of viral enzymes, nonenzymatic viral proteins, and host factors. Many of the nonenzymatic viral proteins, such as NS4B, are associated with the endoplasmic reticulum membrane. How these membrane proteins function in viral replication is poorly understood. Here we report a robust method to express and purify dengue virus (DENV) and West Nile virus NS4B proteins. The NS4B proteins were expressed in Escherich ...[more]