Fitness and virulence of a coxsackievirus mutant that can circumnavigate the need for phosphatidylinositol 4-kinase class III beta.
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ABSTRACT:
SUBMITTER: Thibaut HJ
PROVIDER: S-EPMC3958056 | biostudies-literature | 2014 Mar
REPOSITORIES: biostudies-literature
Publications
Fitness and virulence of a coxsackievirus mutant that can circumnavigate the need for phosphatidylinositol 4-kinase class III beta.
Thibaut Hendrik Jan HJ van der Schaar Hilde M HM Lanke Kjerstin H W KH Verbeken Erik E Andrews Martin M Leyssen Pieter P Neyts Johan J van Kuppeveld Frank J M FJ
Journal of virology 20131226 5
Coxsackieviruses require phosphatidylinositol-4-kinase IIIβ (PI4KIIIβ) for replication but can bypass this need by an H57Y mutation in protein 3A (3A-H57Y). We show that mutant coxsackievirus is not outcompeted by wild-type virus during 10 passages in vitro. In mice, the mutant virus proved as virulent as wild-type virus, even when mice were treated with a PI4KIIIβ inhibitor. Our data suggest that upon emergence, the 3A-H57Y mutant has the fitness to establish a resistant population with a virul ...[more]
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