Polymorphism and resolution of oncogene promoter quadruplex-forming sequences.
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ABSTRACT:
SUBMITTER: Miller MC
PROVIDER: S-EPMC3962748 | biostudies-literature | 2011 Oct
REPOSITORIES: biostudies-literature
Publications
Polymorphism and resolution of oncogene promoter quadruplex-forming sequences.
Miller M Clarke MC Le Huy T HT Dean William L WL Holt Patrick A PA Chaires Jonathan B JB Trent John O JO
Organic & biomolecular chemistry 20110921 22
We report the separation of several quadruplex species formed by ten promoter sequences by Size Exclusion Chromatography (SEC). Modification at the 5' or 3' ends or in loop regions of quadruplex forming sequences has become the standard technique for dealing with quadruplex polymorphism. However, conformations produced employing this method or by other means of artificially shifting the equilibrium may not represent the species that are present in vivo. This method enables an unperturbed view of ...[more]
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