In vitro expansion and genetic modification of gastrointestinal stem cells in spheroid culture.
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ABSTRACT:
SUBMITTER: Miyoshi H
PROVIDER: S-EPMC3969856 | biostudies-literature | 2013 Dec
REPOSITORIES: biostudies-literature
Publications
In vitro expansion and genetic modification of gastrointestinal stem cells in spheroid culture.
Miyoshi Hiroyuki H Stappenbeck Thaddeus S TS
Nature protocols 20131114 12
It is useful to be able to grow enriched populations of stem cells in vitro. Growth of stem cells as tissue spheroids is a key methodology permitting sustainable culture of adult epithelial cells. Gastrointestinal stem cells can be propagated by using conditioned medium from a supportive cell line (L-WRN). This protocol describes how to prepare conditioned medium and how to culture stem cell-enriched epithelial spheroids from the mouse gastrointestine. These spheroids are also amenable to geneti ...[more]
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