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Detection of vanC1 gene transcription in vancomycin-susceptible Enterococcus faecalis.


ABSTRACT: Here we report the presence and expression levels of the vanC1 and vanC(2/3) genes in vancomycin-susceptible strains of Enterococcus faecalis. The vanC1 and vanC(2/3) genes were located in the plasmid DNA and on the chromosome, respectively. Specific mRNA of the vanC1 gene was detected in one of these strains. Additionally, analysis of the vanC gene sequences showed that these genes are related to the vanC genes of Enterococcus gallinarum and Enterococcus casseliflavus. The presence of vanC genes is useful for the identification of E. gallinarum and E. casseliflavus. Moreover, this is the first report of vanC mRNA in E. faecalis.

SUBMITTER: Moura TM 

PROVIDER: S-EPMC3970631 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature

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Detection of vanC1 gene transcription in vancomycin-susceptible Enterococcus faecalis.

Moura Tiane Martin de TM   Cassenego Ana Paula Vaz AP   Campos Fabrício Souza FS   Ribeiro Andrea Machado Leal AM   Franco Ana Cláudia AC   d'Azevedo Pedro Alves PA   Frazzon Jeverson J   Frazzon Ana Paula Guedes AP  

Memorias do Instituto Oswaldo Cruz 20130601 4


Here we report the presence and expression levels of the vanC1 and vanC(2/3) genes in vancomycin-susceptible strains of Enterococcus faecalis. The vanC1 and vanC(2/3) genes were located in the plasmid DNA and on the chromosome, respectively. Specific mRNA of the vanC1 gene was detected in one of these strains. Additionally, analysis of the vanC gene sequences showed that these genes are related to the vanC genes of Enterococcus gallinarum and Enterococcus casseliflavus. The presence of vanC gene  ...[more]

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