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Functions and mechanics of dynein motor proteins.

ABSTRACT: Fuelled by ATP hydrolysis, dyneins generate force and movement on microtubules in a wealth of biological processes, including ciliary beating, cell division and intracellular transport. The large mass and complexity of dynein motors have made elucidating their mechanisms a sizable task. Yet, through a combination of approaches, including X-ray crystallography, cryo-electron microscopy, single-molecule assays and biochemical experiments, important progress has been made towards understanding how these giant motor proteins work. From these studies, a model for the mechanochemical cycle of dynein is emerging, in which nucleotide-driven flexing motions within the AAA+ ring of dynein alter the affinity of its microtubule-binding stalk and reshape its mechanical element to generate movement.

SUBMITTER: Roberts AJ

PROVIDER: S-EPMC3972880 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature

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Functions and mechanics of dynein motor proteins.

Roberts Anthony J AJ Kon Takahide T Knight Peter J PJ Sutoh Kazuo K Burgess Stan A SA

Nature reviews. Molecular cell biology 20130925 11

Fuelled by ATP hydrolysis, dyneins generate force and movement on microtubules in a wealth of biological processes, including ciliary beating, cell division and intracellular transport. The large mass and complexity of dynein motors have made elucidating their mechanisms a sizable task. Yet, through a combination of approaches, including X-ray crystallography, cryo-electron microscopy, single-molecule assays and biochemical experiments, important progress has been made towards understanding how ...[more]

PMID: 24064538

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