Activation of neutrophils by autocrine IL-17A-IL-17RC interactions during fungal infection is regulated by IL-6, IL-23, ROR?t and dectin-2.

ABSTRACT: Here we identified a population of bone marrow neutrophils that constitutively expressed the transcription factor ROR?t and produced and responded to interleukin 17A (IL-17A (IL-17)). IL-6, IL-23 and ROR?t, but not T cells or natural killer (NK) cells, were required for IL-17 production in neutrophils. IL-6 and IL-23 induced expression of the receptors IL-17RC and dectin-2 on neutrophils, and IL-17RC expression was augmented by activation of dectin-2. Autocrine activity of IL-17A and its receptor induced the production of reactive oxygen species (ROS), and increased fungal killing in vitro and in a model of Aspergillus-induced keratitis. Human neutrophils also expressed ROR?t and induced the expression of IL-17A, IL-17RC and dectin-2 following stimulation with IL-6 and IL-23. Our findings identify a population of human and mouse neutrophils with autocrine IL-17 activity that probably contribute to the etiology of microbial and inflammatory diseases.

SUBMITTER: Taylor PR 

PROVIDER: S-EPMC3972892 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature