Cytokine signaling-1 suppressor is inducible by IL-1beta and inhibits the catabolic effects of IL-1beta in chondrocytes: its implication in the paradoxical joint-protective role of IL-1beta.

ABSTRACT: Although IL-1? is believed to be crucial in the pathogenesis of osteoarthritis (OA), the IL-1? blockade brings no therapeutic benefit in human OA and results in OA aggravation in several animal models. We explored the role of a cytokine signaling 1 (SOCS1) suppressor as a regulatory modulator of IL-1? signaling in chondrocytes.Cartilage samples were obtained from patients with knee OA and those without OA who underwent surgery for femur-neck fracture. SOCS1 expression in cartilage was assessed with immunohistochemistry. IL-1?-induced SOCS1 expression in chondrocytes was analyzed with quantitative polymerase chain reaction and immunoblot. The effect of SOCS1 on IL-1? signaling pathways and the synthesis of matrix metalloproteinases (MMPs) and aggrecanase-1 was investigated in SOCS1-overexpressing or -knockdown chondrocytes.SOCS1 expression was significantly increased in OA cartilage, especially in areas of severe damage (P?

SUBMITTER: Choi YS 

PROVIDER: S-EPMC3979110 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature