Unknown

Dataset Information

0

Regulation of experimental autoimmune encephalomyelitis by TPL-2 kinase.


ABSTRACT: Tumor progression locus 2 (TPL-2) expression is required for efficient polarization of naive T cells to Th1 effector cells in vitro, as well as for Th1-mediated immune responses. In the present study, we investigated the potential role of TPL-2 in Th17 cells. TPL-2 was found to be dispensable for Th17 cell differentiation in vitro, and for the initial priming of Th17 cells in experimental autoimmune encephalomyelitis (EAE), a Th17 cell-mediated disease model for multiple sclerosis. Nevertheless, TPL-2-deficient mice were protected from EAE, which correlated with reduced immune cell infiltration, demyelination, and axonal damage in the CNS. Adoptive transfer experiments demonstrated that there was no T cell-intrinsic function for TPL-2 in EAE, and that TPL-2 signaling was not required in radiation-sensitive hematopoietic cells. Rather, TPL-2 signaling in radiation-resistant stromal cells promoted the effector phase of the disease. Importantly, using a newly generated mouse strain expressing a kinase-inactive form of TPL-2, we demonstrated that stimulation of EAE was dependent on the catalytic activity of TPL-2 and not its adaptor function to stabilize the associated ubiquitin-binding protein ABIN-2. Our data therefore raise the possibility that small molecule inhibitors of TPL-2 may be beneficial in multiple sclerosis therapy.

SUBMITTER: Sriskantharajah S 

PROVIDER: S-EPMC3979668 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature

altmetric image

Publications


Tumor progression locus 2 (TPL-2) expression is required for efficient polarization of naive T cells to Th1 effector cells in vitro, as well as for Th1-mediated immune responses. In the present study, we investigated the potential role of TPL-2 in Th17 cells. TPL-2 was found to be dispensable for Th17 cell differentiation in vitro, and for the initial priming of Th17 cells in experimental autoimmune encephalomyelitis (EAE), a Th17 cell-mediated disease model for multiple sclerosis. Nevertheless,  ...[more]

Similar Datasets

| S-EPMC3660233 | biostudies-literature
| S-EPMC6128870 | biostudies-other
| S-EPMC9540342 | biostudies-literature
2024-09-30 | GSE234291 | GEO
| S-EPMC1458883 | biostudies-other
| S-EPMC4402084 | biostudies-other
| S-EPMC3167080 | biostudies-literature
| S-EPMC3397849 | biostudies-literature
| S-EPMC6519352 | biostudies-literature
| S-EPMC3033275 | biostudies-literature