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Crystal structure of the RNA demethylase ALKBH5 from zebrafish.

ABSTRACT: ALKBH5, a member of AlkB family proteins, has been reported as a mammalian N(6)-methyladenosine (m(6)A) RNA demethylase. Here we report the crystal structure of zebrafish ALKBH5 (fALKBH5) with the resolution of 1.65Å. Structural superimposition shows that fALKBH5 is comprised of a conserved jelly-roll motif. However, it possesses a loop that interferes potential binding of a duplex nucleic acid substrate, suggesting an important role in substrate selection. In addition, several active site residues are different between the two known m(6)A RNA demethylases, ALKBH5 and FTO, which may result in their slightly different pathways of m(6)A demethylation.

SUBMITTER: Chen W

PROVIDER: S-EPMC3982313 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature

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Crystal structure of the RNA demethylase ALKBH5 from zebrafish.

Chen Weizhong W Zhang Liang L Zheng Guanqun G Fu Ye Y Ji Quanjiang Q Liu Fange F Chen Hao H He Chuan C

FEBS letters 20140220 6

ALKBH5, a member of AlkB family proteins, has been reported as a mammalian N(6)-methyladenosine (m(6)A) RNA demethylase. Here we report the crystal structure of zebrafish ALKBH5 (fALKBH5) with the resolution of 1.65Å. Structural superimposition shows that fALKBH5 is comprised of a conserved jelly-roll motif. However, it possesses a loop that interferes potential binding of a duplex nucleic acid substrate, suggesting an important role in substrate selection. In addition, several active site resid ...[more]

PMID: 24561204

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Crystal structures of the human RNA demethylase Alkbh5 reveal basis for substrate recognition.

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