Ontology highlight
ABSTRACT:
SUBMITTER: Smith SE
PROVIDER: S-EPMC3983026 | biostudies-literature | 2014 Mar
REPOSITORIES: biostudies-literature
Smith Sara E SE Williams Jessica M JM Ando Shin S Koide Kazunori K
Analytical chemistry 20140214 5
The concentration of human serum albumin (HSA) indicates the health state of individuals and is routinely measured by UV spectroscopy with bromocresol. However, this method tends to overestimate HSA, and more critically, depends highly on the timing, in seconds, of the measurements. Here, we report an analog of 2',7'-dichlorofluorescein that can be used as a fluorescent sensor to quantify HSA in human sera. The accuracy of this new method proved superior to that of bromocresol when an internatio ...[more]