Ontology highlight
ABSTRACT:
SUBMITTER: Yang J
PROVIDER: S-EPMC3688735 | biostudies-other | 2013
REPOSITORIES: biostudies-other
PloS one 20130620 6
Herein, we describe the generation of a monomeric large Stokes shift (LSS) red fluorescent protein, mBeRFP, with excitation and emission peaks at 446 and 615 nm, respectively. Compared with two previously reported LSS-RFPs (mKeima and LSS-mKate2), mBeRFP is approximately three times brighter. In addition, mBeRFP is characterized by improved photostability, rapid maturation, an extended lifetime, and a monomeric nature. Additionally, mBeRFP can be paired with the Alexa 647 dye as a FRET donor to ...[more]