Dataset Information

A dark-light transition triggers expression of the floral promoter CrFTL1 and downregulates CONSTANS-like genes in a short-day plant Chenopodium rubrum.

ABSTRACT: The proper timing of flowering is essential for the adaptation of plant species to their ever-changing environments. The central position in a complex regulatory network is occupied by the protein FT, which acts as a florigen. We found that light, following a permissive period of darkness, was essential to induce the floral promoter CrFTL1 and to initiate flowering in seedlings of the short-day plant Chenopodium rubrum L. We also identified two novel CONSTANS-like genes in C. rubrum and observed their rhythmic diurnal and circadian expressions. Strong rhythmicity of expression suggested that the two genes might have been involved in the regulation of photoperiod-dependent processes, despite their inability to complement co mutation in A. thaliana. The CrCOL1 and CrCOL2 genes were downregulated by dark-light transition, regardless of the length of a preceding dark period. The same treatment activated the floral promoter CrFTL1. Light therefore affected CrCOL and CrFTL1 in an opposite manner. Both CrCOL genes and CrFTL1 displayed expression patterns unique among short-day plants. Chenopodium rubrum, the subject of classical physiological studies in the past, is emerging as a useful model for the investigation of flowering at the molecular level.

SUBMITTER: Drabesova J

PROVIDER: S-EPMC3991744 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature

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A dark-light transition triggers expression of the floral promoter CrFTL1 and downregulates CONSTANS-like genes in a short-day plant Chenopodium rubrum.

Drabešová Jana J Cháb David D Kolař Jan J Haškovcová Kateřina K Štorchová Helena H

Journal of experimental botany 20140318 8

The proper timing of flowering is essential for the adaptation of plant species to their ever-changing environments. The central position in a complex regulatory network is occupied by the protein FT, which acts as a florigen. We found that light, following a permissive period of darkness, was essential to induce the floral promoter CrFTL1 and to initiate flowering in seedlings of the short-day plant Chenopodium rubrum L. We also identified two novel CONSTANS-like genes in C. rubrum and observed ...[more]

PMID: 24642846

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Project description:Photoperiod-regulated floral transition is vital to the flowering plant. Luculia gratissima "Xiangfei" is a flowering ornamental plant with high development potential economically and is a short-day woody perennial. However, the genetic regulation of short-day-induced floral transition in L. gratissima is unclear. To systematically research the responses of L. gratissima during this process, dynamic changes in morphology, physiology, and transcript levels were observed and identified in different developmental stages of long-day- and short-day-treated L. gratissima plants. We found that floral transition in L. gratissima occurred 10 d after short-day induction, but flower bud differentiation did not occur at any stage under long-day conditions. A total of 1,226 differentially expressed genes were identified, of which 146 genes were associated with flowering pathways of sugar, phytohormones, photoperiod, ambient temperature, and aging signals, as well as floral integrator and meristem identity genes. The trehalose-6-phosphate signal positively modulated floral transition by interacting with SQUAMOSA PROMOTER-BINDING-LIKE PROTEIN 4 (SPL4) in the aging pathway. Endogenous gibberellin, abscisic acid, cytokinin, and jasmonic acid promoted floral transition, whereas strigolactone inhibited it. In the photoperiod pathway, FD, CONSTANS-LIKE 12, and nuclear factors Y positively controlled floral transition, whereas PSEUDO-RESPONSE REGULATOR 7, FLAVIN-BINDING KELCH REPEAT F-BOX PROTEIN 1, and LUX negatively regulated it. SPL4 and pEARLI1 positively affected floral transition. Suppressor of Overexpression of Constans 1 and AGAMOUSLIKE24 integrated multiple flowering signals to modulate the expression of FRUITFULL/AGL8, AP1, LEAFY, SEPALLATAs, SHORT VEGETATIVE PHASE, and TERMINAL FLOWER 1, thereby regulating floral transition. Finally, we propose a regulatory network model for short-day-induced floral transition in L. gratissima. This study improves our understanding of flowering time regulation in L. gratissima and provides knowledge for its production and commercialization.

Dataset Information

A dark-light transition triggers expression of the floral promoter CrFTL1 and downregulates CONSTANS-like genes in a short-day plant Chenopodium rubrum.

Publications

A dark-light transition triggers expression of the floral promoter CrFTL1 and downregulates CONSTANS-like genes in a short-day plant Chenopodium rubrum.

Similar Datasets

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