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Multiplex high-resolution melting analysis as a diagnostic tool for detection of plasmid-mediated AmpC ?-lactamase genes.

ABSTRACT: High-resolution melting (HRM) analysis can be a diagnostic tool to evaluate the presence of resistance genes with the added bonus of discriminating sequence modifications. A real-time, multiplex PCR assay using HRM was designed for the detection of plasmid-mediated ampC genes. The specificity and sensitivity of this assay were 96% and 100%, respectively.

SUBMITTER: Geyer CN 

PROVIDER: S-EPMC3993522 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature

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Multiplex high-resolution melting analysis as a diagnostic tool for detection of plasmid-mediated AmpC β-lactamase genes.

Geyer Chelsie N CN   Hanson Nancy D ND  

Journal of clinical microbiology 20140129 4

High-resolution melting (HRM) analysis can be a diagnostic tool to evaluate the presence of resistance genes with the added bonus of discriminating sequence modifications. A real-time, multiplex PCR assay using HRM was designed for the detection of plasmid-mediated ampC genes. The specificity and sensitivity of this assay were 96% and 100%, respectively. ...[more]

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