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Development of a TaqMan multiplex PCR assay for detection of plasmid-mediated ampC ?-lactamase genes.

ABSTRACT: A multiplex, real-time TaqMan assay was designed to identify clinical isolates carrying plasmid-mediated ampC genes. The specificity and sensitivity of this assay were 100% when testing characterized AmpC/non-AmpC-producing isolates and randomly selected clinical isolates. This is a rapid assay that can be performed in a clinical microbiology laboratory.

SUBMITTER: Geyer CN 

PROVIDER: S-EPMC3486237 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature

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Development of a TaqMan multiplex PCR assay for detection of plasmid-mediated ampC β-lactamase genes.

Geyer Chelsie N CN   Reisbig Mark D MD   Hanson Nancy D ND  

Journal of clinical microbiology 20120815 11

A multiplex, real-time TaqMan assay was designed to identify clinical isolates carrying plasmid-mediated ampC genes. The specificity and sensitivity of this assay were 100% when testing characterized AmpC/non-AmpC-producing isolates and randomly selected clinical isolates. This is a rapid assay that can be performed in a clinical microbiology laboratory. ...[more]

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