Naturally occurring mutations in the PA gene are key contributors to increased virulence of pandemic H1N1/09 influenza virus in mice.
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ABSTRACT:
SUBMITTER: Sun Y
PROVIDER: S-EPMC3993729 | biostudies-literature | 2014 Apr
REPOSITORIES: biostudies-literature
Publications
Naturally occurring mutations in the PA gene are key contributors to increased virulence of pandemic H1N1/09 influenza virus in mice.
Sun Yipeng Y Xu Qi Q Shen Ye Y Liu Linqing L Wei Kai K Sun Honglei H Pu Juan J Chang Kin-Chow KC Liu Jinhua J
Journal of virology 20140212 8
We examined the molecular basis of virulence of pandemic H1N1/09 influenza viruses by reverse genetics based on two H1N1/09 virus isolates (A/California/04/2009 [CA04] and A/swine/Shandong/731/2009 [SD731]) with contrasting pathogenicities in mice. We found that four amino acid mutations (P224S in the PA protein [PA-P224S], PB2-T588I, NA-V106I, and NS1-I123V) contributed to the lethal phenotype of SD731. In particular, the PA-P224S mutation when combined with PA-A70V in CA04 drastically reduced ...[more]
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