Novel MPDZ/MUPP1 transgenic and knockdown models confirm Mpdz's role in ethanol withdrawal and support its role in voluntary ethanol consumption.
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ABSTRACT:
SUBMITTER: Milner LC
PROVIDER: S-EPMC3997615 | biostudies-literature | 2015 Jan
REPOSITORIES: biostudies-literature
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Novel MPDZ/MUPP1 transgenic and knockdown models confirm Mpdz's role in ethanol withdrawal and support its role in voluntary ethanol consumption.
Milner Lauren C LC Shirley Renee L RL Kozell Laura B LB Walter Nicole A NA Kruse Lauren C LC Komiyama Noboru H NH Grant Seth G N SG Buck Kari J KJ
Addiction biology 20131010 1
Association studies implicate multiple PDZ domain protein (MPDZ/MUPP1) sequence and/or expression in risk for alcoholism in humans and ethanol withdrawal (EW) in mice, but confirmation has been hindered by the dearth of targeted genetic models. We report the creation of transgenic (MPDZ-TG) and knockout heterozygote (Mpdz(+/-) ) mice, with increased (2.9-fold) and decreased (53%) target expression, respectively. Both models differ in EW compared with wild-type littermates (P ≤ 0.03), providing c ...[more]
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