Ontology highlight
ABSTRACT:
SUBMITTER: Chen J
PROVIDER: S-EPMC3999860 | biostudies-literature | 2014 Apr
REPOSITORIES: biostudies-literature
Chen Jia J Miller Brendan F BF Furano Anthony V AV
eLife 20140429
'Normal' genomic DNA contains hundreds of mismatches that are generated daily by the spontaneous deamination of C (U/G) and methyl-C (T/G). Thus, a mutagenic effect of their repair could constitute a serious genetic burden. We show here that while mismatches introduced into human cells on an SV40-based episome were invariably repaired, this process induced mutations in flanking DNA at a significantly higher rate than no mismatch controls. Most mutations involved the C of TpC, the substrate of so ...[more]