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Bovine lactoferrin-derived peptides as novel broad-spectrum inhibitors of influenza virus.

ABSTRACT: Bovine lactoferrin (bLf) is a multifunctional glycoprotein that plays an important role in innate immunity against infections, including influenza. Here we have dissected bLf into its C- and N-lobes and show that inhibition of influenza virus hemagglutination and cell infection is entirely attributable to the C-lobe and that all major virus subtypes, including H1N1 and H3N2, are inhibited. By far-western blotting and sequencing studies, we demonstrate that bLf C-lobe strongly binds to the HA(2) region of viral hemagglutinin, precisely the highly conserved region containing the fusion peptide. By molecular docking studies, three C-lobe fragments were identified which inhibited virus hemagglutination and infection at fentomolar concentration range. Besides contributing to explain the broad anti-influenza activity of bLf, our findings lay the foundations for exploiting bLf fragments as source of potential anti-influenza therapeutics.

SUBMITTER: Ammendolia MG

PROVIDER: S-EPMC4001507 | biostudies-literature | 2012 Mar

REPOSITORIES: biostudies-literature

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Bovine lactoferrin-derived peptides as novel broad-spectrum inhibitors of influenza virus.

Ammendolia Maria Grazia MG Agamennone Mariangela M Pietrantoni Agostina A Lannutti Fabio F Siciliano Rosa Anna RA De Giulio Beatrice B Amici Carla C Superti Fabiana F

Pathogens and global health 20120301 1

Bovine lactoferrin (bLf) is a multifunctional glycoprotein that plays an important role in innate immunity against infections, including influenza. Here we have dissected bLf into its C- and N-lobes and show that inhibition of influenza virus hemagglutination and cell infection is entirely attributable to the C-lobe and that all major virus subtypes, including H1N1 and H3N2, are inhibited. By far-western blotting and sequencing studies, we demonstrate that bLf C-lobe strongly binds to the HA(2) ...[more]

PMID: 22595270

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Bovine lactoferrin-derived peptides as novel broad-spectrum inhibitors of influenza virus.

Publications

Bovine lactoferrin-derived peptides as novel broad-spectrum inhibitors of influenza virus.

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