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Identification of p100 target promoters by chromatin immunoprecipitation-guided ligation and selection (ChIP-GLAS).

ABSTRACT: The multifunctional protein p100 is a vital transcriptional regulator that increases gene transcription by forming a physical bridge between promoter-specific transcription factors and the basal transcription machinery. To identify potential signal transduction pathways in which human p100 acts as a coregulator and to find target promoter regions that may interact with p100, we performed a promoter microarray assay called chromatin immunoprecipitation-guided ligation and selection (ChIP-GLAS). From this assay, we determined that a set of promoter fragments, including several factors in the transforming growth factor beta (TGF-?) signaling pathway, exhibited interaction with p100. The ChIP-GLAS data were validated by RT-PCR assessing the mRNA expression of various factors in the TGF-? signaling pathway in cell lines.

SUBMITTER: Liu X

PROVIDER: S-EPMC4002985 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature

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Identification of p100 target promoters by chromatin immunoprecipitation-guided ligation and selection (ChIP-GLAS).

Liu Xin X Dong Lijie L Zhang Xuejun X Wang Baoya B Wang Xinting X Li Hu H He Jinyan J Ge Lin L Jing Xiang X Yao Zhi Z Yang Jie J

Cellular & molecular immunology 20101004 1

The multifunctional protein p100 is a vital transcriptional regulator that increases gene transcription by forming a physical bridge between promoter-specific transcription factors and the basal transcription machinery. To identify potential signal transduction pathways in which human p100 acts as a coregulator and to find target promoter regions that may interact with p100, we performed a promoter microarray assay called chromatin immunoprecipitation-guided ligation and selection (ChIP-GLAS). F ...[more]

PMID: 20921938

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