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Synthesis of fluorosurfactants for emulsion-based biological applications.

ABSTRACT: Microemulsion represents an attractive platform for fundamental and applied biomedical research because the emulsified droplets can serve as millions of compartmentalized micrometer-sized reactors amenable to high-throughput screening or online monitoring. However, establishing stable emulsions with surfactants that are compatible with biological applications remains a significant challenge. Motivated by the lack of commercially available surfactants suitable for microemulsion-based biological assays, this study describes the facile synthesis of a biocompatible fluorosurfactant with nonionic tris(hydroxymethyl)methyl (Tris) polar head groups. We have further demonstrated compatibility of the developed surfactant with diverse emulsion-based applications, including DNA polymeric nanoparticle synthesis, enzymatic activity assay, and bacterial or mammalian cell culture, in the setup of both double- and multiphases of emulsions.

SUBMITTER: Chiu YL

PROVIDER: S-EPMC4004322 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature

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Synthesis of fluorosurfactants for emulsion-based biological applications.

Chiu Ya-Ling YL Chan Hon Fai HF Phua Kyle K L KK Zhang Ying Y Juul Sissel S Knudsen Birgitta R BR Ho Yi-Ping YP Leong Kam W KW

ACS nano 20140325 4

Microemulsion represents an attractive platform for fundamental and applied biomedical research because the emulsified droplets can serve as millions of compartmentalized micrometer-sized reactors amenable to high-throughput screening or online monitoring. However, establishing stable emulsions with surfactants that are compatible with biological applications remains a significant challenge. Motivated by the lack of commercially available surfactants suitable for microemulsion-based biological a ...[more]

PMID: 24646088

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