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Synthesis of double-clickable functionalised graphene oxide for biological applications.

ABSTRACT: Azide- and alkyne-double functionalised graphene oxide (Click(2) GO) was synthesised and characterised with attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR), thermogravimetric analysis (TGA) and Raman spectroscopy. Fourteen-percentage increase in azide content was found, after pre-treatment of GO with meta-chloroperoxybenzoic acid (mCPBA), determined with elemental analysis. No effect on A549 cell viability was found, up to 100 ?g mL(-1) and 72 h of incubation, determined with the modified lactate dehydrogenase (mLDH) assay. Two sequential copper(i) catalysed azide-alkyne cycloaddition (CuAAC) reactions were performed to conjugate the propargyl-modified blood-brain barrier targeting peptide Angiopep-2, and a bis-azide polyethylene glycol (MW = 3500), to the Click(2) GO. The final conjugate was characterised with ATR-FTIR and TGA.

SUBMITTER: Mei KC

PROVIDER: S-EPMC4594119 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature

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Synthesis of double-clickable functionalised graphene oxide for biological applications.

Mei Kuo-Ching KC Rubio Noelia N Costa Pedro M PM Kafa Houmam H Abbate Vincenzo V Festy Frederic F Bansal Sukhvinder S SS Hider Robert C RC Al-Jamal Khuloud T KT

Chemical communications (Cambridge, England) 20151001 81

Azide- and alkyne-double functionalised graphene oxide (Click(2) GO) was synthesised and characterised with attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR), thermogravimetric analysis (TGA) and Raman spectroscopy. Fourteen-percentage increase in azide content was found, after pre-treatment of GO with meta-chloroperoxybenzoic acid (mCPBA), determined with elemental analysis. No effect on A549 cell viability was found, up to 100 μg mL(-1) and 72 h of incubation, det ...[more]

PMID: 26295072

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