Ontology highlight
ABSTRACT:
SUBMITTER: Hazel J
PROVIDER: S-EPMC4004590 | biostudies-literature | 2013 Nov
REPOSITORIES: biostudies-literature
Hazel James J Krutkramelis Kaspars K Mooney Paul P Tomschik Miroslav M Gerow Ken K Oakey John J Gatlin J C JC
Science (New York, N.Y.) 20131101 6160
The mitotic spindle must function in cell types that vary greatly in size, and its dimensions scale with the rapid, reductive cell divisions that accompany early stages of development. The mechanism responsible for this scaling is unclear, because uncoupling cell size from a developmental or cellular context has proven experimentally challenging. We combined microfluidic technology with Xenopus egg extracts to characterize spindle assembly within discrete, geometrically defined volumes of cytopl ...[more]