Ontology highlight
ABSTRACT:
SUBMITTER: Juillerat A
PROVIDER: S-EPMC4005648 | biostudies-literature | 2014 Apr
REPOSITORIES: biostudies-literature
Juillerat Alexandre A Dubois Gwendoline G Valton Julien J Thomas Séverine S Stella Stefano S Maréchal Alan A Langevin Stéphanie S Benomari Nassima N Bertonati Claudia C Silva George H GH Daboussi Fayza F Epinat Jean-Charles JC Montoya Guillermo G Duclert Aymeric A Duchateau Philippe P
Nucleic acids research 20140224 8
A key issue when designing and using DNA-targeting nucleases is specificity. Ideally, an optimal DNA-targeting tool has only one recognition site within a genomic sequence. In practice, however, almost all designer nucleases available today can accommodate one to several mutations within their target site. The ability to predict the specificity of targeting is thus highly desirable. Here, we describe the first comprehensive experimental study focused on the specificity of the four commonly used ...[more]