Ontology highlight
ABSTRACT:
SUBMITTER: Tan YC
PROVIDER: S-EPMC4006370 | biostudies-literature | 2014 Mar
REPOSITORIES: biostudies-literature
Tan Yann-Chong YC Blum Lisa K LK Kongpachith Sarah S Ju Chia-Hsin CH Cai Xiaoyong X Lindstrom Tamsin M TM Sokolove Jeremy J Robinson William H WH
Clinical immunology (Orlando, Fla.) 20140109 1
We developed a DNA barcoding method to enable high-throughput sequencing of the cognate heavy- and light-chain pairs of the antibodies expressed by individual B cells. We used this approach to elucidate the plasmablast antibody response to influenza vaccination. We show that >75% of the rationally selected plasmablast antibodies bind and neutralize influenza, and that antibodies from clonal families, defined by sharing both heavy-chain VJ and light-chain VJ sequence usage, do so most effectively ...[more]