Functional interrogation and mining of natively paired human VH:VL antibody repertoires.
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ABSTRACT:
SUBMITTER: Wang B
PROVIDER: S-EPMC5801115 | biostudies-literature | 2018 Feb
REPOSITORIES: biostudies-literature
Publications
Functional interrogation and mining of natively paired human V<sub>H</sub>:V<sub>L</sub> antibody repertoires.
Wang Bo B DeKosky Brandon J BJ Timm Morgan R MR Lee Jiwon J Normandin Erica E Misasi John J Kong Rui R McDaniel Jonathan R JR Delidakis George G Leigh Kendra E KE Niezold Thomas T Choi Chang W CW Viox Elise G EG Fahad Ahmed A Cagigi Alberto A Ploquin Aurélie A Leung Kwanyee K Yang Eun Sung ES Kong Wing-Pui WP Voss William N WN Schmidt Aaron G AG Moody M Anthony MA Ambrozak David R DR Henry Amy R AR Laboune Farida F Ledgerwood Julie E JE Graham Barney S BS Connors Mark M Douek Daniel C DC Sullivan Nancy J NJ Ellington Andrew D AD Mascola John R JR Georgiou George G
Nature biotechnology 20180108 2
We present a technology to screen millions of B cells for natively paired human antibody repertoires. Libraries of natively paired, variable region heavy and light (V<sub>H</sub>:V<sub>L</sub>) amplicons are expressed in a yeast display platform that is optimized for human Fab surface expression. Using our method we identify HIV-1 broadly neutralizing antibodies (bNAbs) from an HIV-1 slow progressor and high-affinity neutralizing antibodies against Ebola virus glycoprotein and influenza hemagglu ...[more]
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