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Functional interrogation and mining of natively paired human VH:VL antibody repertoires.

ABSTRACT: We present a technology to screen millions of B cells for natively paired human antibody repertoires. Libraries of natively paired, variable region heavy and light (VH:VL) amplicons are expressed in a yeast display platform that is optimized for human Fab surface expression. Using our method we identify HIV-1 broadly neutralizing antibodies (bNAbs) from an HIV-1 slow progressor and high-affinity neutralizing antibodies against Ebola virus glycoprotein and influenza hemagglutinin.

SUBMITTER: Wang B 

PROVIDER: S-EPMC5801115 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature

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Functional interrogation and mining of natively paired human V<sub>H</sub>:V<sub>L</sub> antibody repertoires.

Wang Bo B   DeKosky Brandon J BJ   Timm Morgan R MR   Lee Jiwon J   Normandin Erica E   Misasi John J   Kong Rui R   McDaniel Jonathan R JR   Delidakis George G   Leigh Kendra E KE   Niezold Thomas T   Choi Chang W CW   Viox Elise G EG   Fahad Ahmed A   Cagigi Alberto A   Ploquin Aurélie A   Leung Kwanyee K   Yang Eun Sung ES   Kong Wing-Pui WP   Voss William N WN   Schmidt Aaron G AG   Moody M Anthony MA   Ambrozak David R DR   Henry Amy R AR   Laboune Farida F   Ledgerwood Julie E JE   Graham Barney S BS   Connors Mark M   Douek Daniel C DC   Sullivan Nancy J NJ   Ellington Andrew D AD   Mascola John R JR   Georgiou George G  

Nature biotechnology 20180108 2

We present a technology to screen millions of B cells for natively paired human antibody repertoires. Libraries of natively paired, variable region heavy and light (V<sub>H</sub>:V<sub>L</sub>) amplicons are expressed in a yeast display platform that is optimized for human Fab surface expression. Using our method we identify HIV-1 broadly neutralizing antibodies (bNAbs) from an HIV-1 slow progressor and high-affinity neutralizing antibodies against Ebola virus glycoprotein and influenza hemagglu  ...[more]

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