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Amino acid changes in the spike protein of feline coronavirus correlate with systemic spread of virus from the intestine and not with feline infectious peritonitis.

ABSTRACT: Recent evidence suggests that a mutation in the spike protein gene of feline coronavirus (FCoV), which results in an amino acid change from methionine to leucine at position 1058, may be associated with feline infectious peritonitis (FIP). Tissue and faecal samples collected post mortem from cats diagnosed with or without FIP were subjected to RNA extraction and quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR) to detect FCoV RNA. In cats with FIP, 95% of tissue, and 81% of faecal samples were PCR-positive, as opposed to 22% of tissue, and 60% of faecal samples in cats without FIP. Relative FCoV copy numbers were significantly higher in the cats with FIP, both in tissues (P?

SUBMITTER: Porter E

PROVIDER: S-EPMC4006447 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature

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Amino acid changes in the spike protein of feline coronavirus correlate with systemic spread of virus from the intestine and not with feline infectious peritonitis.

Porter Emily E Tasker Séverine S Day Michael J MJ Harley Ross R Kipar Anja A Siddell Stuart G SG Helps Christopher R CR

Veterinary research 20140425

Recent evidence suggests that a mutation in the spike protein gene of feline coronavirus (FCoV), which results in an amino acid change from methionine to leucine at position 1058, may be associated with feline infectious peritonitis (FIP). Tissue and faecal samples collected post mortem from cats diagnosed with or without FIP were subjected to RNA extraction and quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR) to detect FCoV RNA. In cats with FIP, 95% of tissue, and 81% of ...[more]

PMID: 24767677

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Limitations of using feline coronavirus spike protein gene mutations to diagnose feline infectious peritonitis.

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Phylogenetic analysis of feline coronavirus strains in an epizootic outbreak of feline infectious peritonitis.

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